Composite

Part:BBa_K2128007:Design

Designed by: David Sanderson   Group: iGEM16_Genspace   (2016-08-13)


GFP with H. Dujardini ACTN Promoter, RBS and Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1218
    Illegal BamHI site found at 997
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI site found at 280


Design Notes

We codon optimized the RFP ORF for C. Elegans as no optimization for H. Dujardini or other Tardigrade species is available.



Source

H. Dujardini, GFP originally from Aequorea victoria.

References